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primary mouse monoclonal gad67  (Merck & Co)

 
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    Structured Review

    Merck & Co primary mouse monoclonal gad67
    Primary Mouse Monoclonal Gad67, supplied by Merck & Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+mouse+monoclonal+gad67/gad67/pm37591355-121-14-17
    Average 90 stars, based on 1 article reviews
    primary mouse monoclonal gad67 - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Immunohistochemistry:

    Article Title: Increased excitability and reduced GABAergic levels in somatosensory cortex under chronic spinal cord injury.
    Article Snippet: Sections were rinsed with PBS three times (10 min each) and then preincubated for 1 h at room temperature in 5% normal donkey serum (SouthernBiotech) and 0.2% Triton X-100 (Sigma-Aldrich) diluted in PBS (blocking solution). .. Immunohistochemistry was performed by incubating the sections overnight at 4 ◦C with the primary mouse monoclonal GAD67 (Merck) (1:1000 in PBS containing 5% normal donkey serum and 0.2% Triton X- 100). .. The following day, sections were washed with PBS three times (10 min each) and incubated for 2 h at room temperature with the donkey anti mouse secondary antibody Alexa 488 (ThermoFisher) (1:1000 in PBS containing 5% normal donkey serum and 0.2% Triton X100).



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    Fluorescence micrographs of coronal brain sections from respective LHA→hM3Dq Cartpt cre/+ mice, showing partial co-expression of the mCherry reporter with <t>GAD67</t> ( A ), and MCH ( B ) (white arrows, right panels), while no co-expression could be observed with orexin ( C ) (right panel). Scale bar = 75 μm.
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    monoclonal mouse anti gad67 primary antibody mab5406  (Merck KGaA)
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    Fluorescence micrographs of coronal brain sections from respective LHA→hM3Dq Cartpt cre/+ mice, showing partial co-expression of the mCherry reporter with <t>GAD67</t> ( A ), and MCH ( B ) (white arrows, right panels), while no co-expression could be observed with orexin ( C ) (right panel). Scale bar = 75 μm.
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    Image Search Results


    Fluorescence micrographs of coronal brain sections from respective LHA→hM3Dq Cartpt cre/+ mice, showing partial co-expression of the mCherry reporter with GAD67 ( A ), and MCH ( B ) (white arrows, right panels), while no co-expression could be observed with orexin ( C ) (right panel). Scale bar = 75 μm.

    Journal: eLife

    Article Title: Arcuate nucleus and lateral hypothalamic CART neurons in the mouse brain exert opposing effects on energy expenditure

    doi: 10.7554/eLife.36494

    Figure Lengend Snippet: Fluorescence micrographs of coronal brain sections from respective LHA→hM3Dq Cartpt cre/+ mice, showing partial co-expression of the mCherry reporter with GAD67 ( A ), and MCH ( B ) (white arrows, right panels), while no co-expression could be observed with orexin ( C ) (right panel). Scale bar = 75 μm.

    Article Snippet: In order to investigate co-expressed transmitters within the hM3Dq-expressing CART neurons immunohistochemistry was also performed for GAD67 (mouse anti-GAD67 monoclonal primary antibody, MAB5406, 1:500, Chemicon International, Temecula, CA, USA), secondary anti-mouse Alexa Fluor 488 antibody (1:500, Molecular Probes, Eugene, OR), MCH (rabbit-anti MCH, 1:1000, Phoenix Pharmaceuticals, Inc., Belmont, CA, USA), secondary anti-rabbit Alexa Fluor 488 antibody (1:500, Molecular Probes, Eugene, OR) and orexin (goat anti-orexin polyclonal primary antibody, sc-8070, 1:1000, Santa Cruz Biotechnology Inc, Santa Cruz, CA, USA), secondary anti-goat Alexa Fluor 488 antibody (1:500, Molecular Probes, Eugene, OR).

    Techniques: Fluorescence, Expressing

    Journal: eLife

    Article Title: Arcuate nucleus and lateral hypothalamic CART neurons in the mouse brain exert opposing effects on energy expenditure

    doi: 10.7554/eLife.36494

    Figure Lengend Snippet:

    Article Snippet: In order to investigate co-expressed transmitters within the hM3Dq-expressing CART neurons immunohistochemistry was also performed for GAD67 (mouse anti-GAD67 monoclonal primary antibody, MAB5406, 1:500, Chemicon International, Temecula, CA, USA), secondary anti-mouse Alexa Fluor 488 antibody (1:500, Molecular Probes, Eugene, OR), MCH (rabbit-anti MCH, 1:1000, Phoenix Pharmaceuticals, Inc., Belmont, CA, USA), secondary anti-rabbit Alexa Fluor 488 antibody (1:500, Molecular Probes, Eugene, OR) and orexin (goat anti-orexin polyclonal primary antibody, sc-8070, 1:1000, Santa Cruz Biotechnology Inc, Santa Cruz, CA, USA), secondary anti-goat Alexa Fluor 488 antibody (1:500, Molecular Probes, Eugene, OR).

    Techniques: SYBR Green Assay, Staining, Immunoprecipitation, Protease Inhibitor, Software